TB-500 Protocol

Dosing & Research

Last Updated: September 4, 2026

Chemical Identity

Amino Acid Sequence: Ac-Ser-Asp-Lys-Pro-Asp-Met-Gly-Glu-Ile-Ala-Ser-Phe-Asp-Lys-Ala-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser (Thymosin Beta-4)
Molecular Formula: C212H350N56O78S
Molecular Weight: 4963.55 g/mol
CAS Number: 77591-33-4

Soft-tissue healing research, Cell migration and actin dynamics, Angiogenesis models, Recovery protocols

Evidence Level

Mixed Evidence

Species Studied

Rat • Mouse • In vitro

Half-Life

~3 Days

Administration

Subcutaneous

Frequency

Split into 2-3 doses per week

How It Works

Soft-tissue healing research
Cell migration and actin dynamics
Angiogenesis models
Recovery protocols

Dosing Structure

Dose

500 mcg daily

Frequency

Split into 2-3 doses per week

Notes

Loading phase (weeks 1-4): 2–5mg per week, split into 2-3 doses. Maintenance phase: 2mg per week. Cycle: 8-12 weeks. Inject: Belly, thigh, shoulder (SubQ) or larger muscles (IM). Flexible timing. Break: 4-6 weeks.

Example Protocols

Dose: 500 mcg daily

Frequency: Split into 2-3 doses per week

Duration: 6-12 weeks

Off Period: Not listed

Calculate dosing →

Find Research Grade TB-500

Recommended vendors for sourcing research-grade material. PeptaBase does not sell peptides directly.

Vendor One Research Labs

COA Verified

Third-party COA-verified research peptides with published batch testing.

Source Coming Soon

Vendor Two Sciences

Purity Tested

US-based research compound supplier with independent purity testing.

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Vendor Three Peptide Co.

Bulk research peptide sourcing with batch-level documentation.

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View all recommended vendors →

Protocol Notes

•

Loading phase (weeks 1-4): 2–5mg per week, split into 2-3 doses.

•

Maintenance phase: 2mg per week.

•

Cycle: 8-12 weeks.

•

Inject: Belly, thigh, shoulder (SubQ) or larger muscles (IM).

•

Flexible timing.

•

Break: 4-6 weeks.

What Is TB-500?

TB-500 is a synthetic analogue of the active region of Thymosin Beta-4 - a protein naturally produced by platelets and found throughout the body. It regulates actin, the scaffold protein inside every cell that enables movement and repair. Researchers study TB-500 for accelerating wound healing, reducing inflammation, and potentially supporting cardiac and neurological tissue repair.

Research Observations - Not Medical Claims

▸Thymosin Beta-4 promoted cardiomyocyte survival and reduced infarct size by ~24% in a mouse myocardial infarction model (PMID: 22959979).
▸Hair follicle activation: TB-4 (full protein) stimulated hair regrowth in mouse dorsal skin models; 20% faster follicle entry into anagen phase vs. controls.
▸Corneal healing: Topical thymosin beta-4 accelerated corneal epithelial wound closure in rabbit models by approximately 40% vs. saline (PMID: 12740574).
▸Central nervous system: Thymosin beta-4 improved motor function recovery and promoted oligodendrocyte differentiation in mouse stroke models.
▸Note: Most studies use full-length thymosin beta-4 protein, not the TB-500 fragment specifically. The two are often conflated in non-peer-reviewed literature.