TB-500 Protocol
Dosing & Research
Last Updated: April 22, 2026
Soft-tissue healing research, Cell migration and actin dynamics, Angiogenesis models, Recovery protocols
Evidence Level
Mixed Evidence
Species Studied
Rat • Mouse • In vitro
Half-Life
~3 Days
Administration
Subcutaneous
Frequency
Split into 2-3 doses per week
How It Works
- Soft-tissue healing research
- Cell migration and actin dynamics
- Angiogenesis models
- Recovery protocols
Dosing Structure
Dose
500 mcg daily
Frequency
Split into 2-3 doses per week
Notes
Loading phase (weeks 1-4): 2–5mg per week, split into 2-3 doses. Maintenance phase: 2mg per week. Cycle: 8-12 weeks. Inject: Belly, thigh, shoulder (SubQ) or larger muscles (IM). Flexible timing. Break: 4-6 weeks.
Example Protocols
Dose: 500 mcg daily
Frequency: Split into 2-3 doses per week
Duration: 6-12 weeks
Off Period: Not listed
Protocol Notes
•
Loading phase (weeks 1-4): 2–5mg per week, split into 2-3 doses.
•
Maintenance phase: 2mg per week.
•
Cycle: 8-12 weeks.
•
Inject: Belly, thigh, shoulder (SubQ) or larger muscles (IM).
•
Flexible timing.
•
Break: 4-6 weeks.
Research & Studies
Loading citations…
- Curated References
- CuratedPubMedPMID 22959979Thymosin beta 4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair↗Nature · 2012 · Bock-Marquette I, Saxena A, White MD, et al.mechanismcardiacanimal
- CuratedPubMedPMID 22492621Thymosin beta 4 promotes tissue repair and modulates inflammation in mouse corneal wound healing↗Exp Eye Res · 2012 · Sosne G, Qiu P, Ousler GW 3rd.wound healinganimal
- CuratedPubMedPMID 21620466Thymosin beta 4 sulfoxide is an anti-inflammatory agent generated by monocytes in the presence of glucocorticoids↗Nat Med · 1999 · Young JD, Lawrence AJ, MacLennan AG, et al.anti-inflammatorymechanism
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