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Healing & Recovery

DSIP Protocol

Dosing & Research

Last Updated: September 4, 2026

Chemical Identity
Amino Acid Sequence
Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu
Molecular Formula
C35H48N10O15
Molecular Weight
848.81 g/mol
CAS Number
62568-57-4

Sleep architecture studies, Stress response modulation, Analgesia research, Neuroendocrine regulation

Evidence Level
Mixed Evidence
Species Studied
Human • Rat • Rabbit
Half-Life
Approximately 7-15 minutes in plasma/in vitro
Administration
• Subcutaneous • Intranasal
Frequency
As needed, 2-3 hours before bed

How It Works

  • Sleep architecture studies
  • Stress response modulation
  • Analgesia research
  • Neuroendocrine regulation

Dosing Structure

Dose
250 mcg
Frequency
As needed, 2-3 hours before bed
Notes
SubQ Injection: 100-300mcg as needed, approximately 2-3 hours before bed. Nasal Spray: 100-150mcg per nostril, as needed, approximately 2-3 hours before bed. No standard cycle duration; some sources recommend 4 weeks on 4 weeks off, others suggest 8 weeks on 4 weeks off. Exercise judgment. Break: 2-4 weeks between cycles.

Example Protocols

Dose: 250 mcg
Frequency: As needed, 2-3 hours before bed
Duration: 6-8 weeks
Off Period: Not listed
Calculate dosing →

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Protocol Notes

•
SubQ Injection: 100-300mcg as needed, approximately 2-3 hours before bed.
•
Nasal Spray: 100-150mcg per nostril, as needed, approximately 2-3 hours before bed.
•
No standard cycle duration; some sources recommend 4 weeks on 4 weeks off, others suggest 8 weeks on 4 weeks off.
•
Exercise judgment.
•
Break: 2-4 weeks between cycles.

What Is DSIP?

DSIP (Delta Sleep-Inducing Peptide) is a nonapeptide (9 amino acids) first isolated from rabbit cerebral venous blood in 1977. It was named for its ability to induce delta-wave (slow-wave) sleep - the deepest, most restorative stage of sleep - in rabbits when infused into the brain. Beyond sleep, it modulates cortisol and LH release, reduces stress hormones, and shows neuroprotective effects in animal models.

Research Observations - Not Medical Claims
  • ▸Sleep induction: Original discovery (1977) showed intraventricular DSIP in rabbits induced synchronized delta-wave EEG patterns - sleep stage 3/4 induction (PMID: 904988).
  • ▸Human sleep studies: IV DSIP (25 nmol/kg) improved sleep efficiency and reduced early morning awakening in patients with chronic insomnia in Swiss research (1983–1988).
  • ▸Stress hormone modulation: DSIP reduces ACTH and corticotropin release; studies show attenuated cortisol responses to stress in human subjects after DSIP administration.
  • ▸LH modulation: DSIP influences luteinizing hormone (LH) release - suggesting a role in neuroendocrine regulation beyond sleep.
  • ▸Neuroprotection: Animal studies show DSIP protects against ethanol-induced neurodegeneration and improves cognitive performance in aged rodents.

Evidence Summary

Mixed EvidenceResearch Chemical - not approved for human therapeutic use in any jurisdiction
Evidence StrengthPreliminary
PreliminaryModerateStrong
Curated Citations2
Largest Study (n)30
Most Recent Study2021
Species Studied
HumanRatRabbit

Commonly Researched With

Epithalon
Both are studied in sleep and longevity contexts - DSIP for delta-wave sleep promotion, Epithalon for circadian and pineal regulation.
Selank
Selank's anxiolytic effects complement DSIP's sleep-inducing activity in stress and sleep quality research protocols.
BPC-157
BPC-157's CNS modulatory properties and DSIP's neuroendocrine effects are studied together in stress recovery and neurological research.

Research & Studies

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