NAD+ Protocol

Dosing & Research

Last Updated: April 22, 2026

Cellular energy metabolism, Sirtuin-related research, Mitochondrial function, Age-related metabolic decline

Evidence Level

Mixed Evidence

Species Studied

Human • Mouse • Rat

Half-Life

Route-dependent; not simply summarized by single plasma value

Administration

Subcutaneous

Frequency

2-3x weekly (titrated up)

How It Works

Cellular energy metabolism
Sirtuin-related research
Mitochondrial function
Age-related metabolic decline

Dosing Structure

Dose

50–100 mg per session

Frequency

2-3x weekly (titrated up)

Notes

SubQ titration: Week 1-2: 25mg 2x per week (may feel lethargic as body adjusts to increased NAD levels) | Week 3-4: 50mg 2x per week | Week 5-6: 50mg 3x per week | Week 7+: may continue to increase as needed until reaching a max of 100mg 3x per week. Inject: SubQ abdomen/thigh. Morning preferred. Break: 4-8 weeks between intensive cycles.

Example Protocols

Dose: 50–100 mg per session

Frequency: 2-3x weekly (titrated up)

Duration: Ongoing

Off Period: Not listed

Log Injection Add to Protocol Calculate dosing →

Protocol Notes

SubQ titration

Week 1-2: 25mg 2x per week (may feel lethargic as body adjusts to increased NAD levels)Week 3-4: 50mg 2x per weekWeek 5-6: 50mg 3x per weekWeek 7+: may continue to increase as needed until reaching a max of 100mg 3x per week

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Inject: SubQ abdomen/thigh.

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Morning preferred.

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Break: 4-8 weeks between intensive cycles.