NAD+ Protocol

Dosing & Research

Last Updated: September 4, 2026

Chemical Identity

Amino Acid Sequence: N/A - dinucleotide, not a peptide
Molecular Formula: C21H27N7O14P2
Molecular Weight: 663.43 g/mol
CAS Number: 53-84-9

Cellular energy metabolism, Sirtuin-related research, Mitochondrial function, Age-related metabolic decline

Evidence Level

Mixed Evidence

Species Studied

Human • Mouse • Rat

Half-Life

Route-dependent; not simply summarized by single plasma value

Administration

Subcutaneous

Frequency

2-3x weekly (titrated up)

How It Works

Cellular energy metabolism
Sirtuin-related research
Mitochondrial function
Age-related metabolic decline

Dosing Structure

Dose

50–100 mg per session

Frequency

2-3x weekly (titrated up)

Notes

SubQ titration: Week 1-2: 25mg 2x per week (may feel lethargic as body adjusts to increased NAD levels) | Week 3-4: 50mg 2x per week | Week 5-6: 50mg 3x per week | Week 7+: may continue to increase as needed until reaching a max of 100mg 3x per week. Inject: SubQ abdomen/thigh. Morning preferred. Break: 4-8 weeks between intensive cycles.

Example Protocols

Dose: 50–100 mg per session

Frequency: 2-3x weekly (titrated up)

Duration: Ongoing

Off Period: Not listed

Calculate dosing →

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Protocol Notes

SubQ titration

Week 1-2: 25mg 2x per week (may feel lethargic as body adjusts to increased NAD levels)Week 3-4: 50mg 2x per weekWeek 5-6: 50mg 3x per weekWeek 7+: may continue to increase as needed until reaching a max of 100mg 3x per week

•

Inject: SubQ abdomen/thigh.

•

Morning preferred.

•

Break: 4-8 weeks between intensive cycles.

What Is NAD+?

NAD+ (nicotinamide adenine dinucleotide) is a coenzyme present in every cell that acts as an electron carrier in energy production and as a substrate for enzymes like sirtuins and PARP that govern DNA repair and stress response. NAD+ levels decline approximately 50% between ages 40–60 in multiple tissues. Supplementation research explores whether restoring NAD+ levels can slow aging-related decline in metabolism, cognition, and cellular integrity.

Research Observations - Not Medical Claims

▸NR supplementation (Elysium Basis trial, 2018; PMID: 29184669): 250 mg and 500 mg NR daily raised whole-blood NAD+ by 40% and 90% respectively over 8 weeks in healthy older adults - first confirmed human evidence that oral NAD+ precursors raise systemic NAD+.
▸NMN in older adults (Yamaguchi et al., 2022; PMID: 34912960): 250 mg/day oral NMN for 12 weeks significantly increased blood NAD+ and improved muscle performance metrics in older men.
▸IV NAD+ (case series, Birkmayer): Intravenous NAD+ infusion (500–1000 mg) acutely raises blood NAD+ to supraphysiological levels; used clinically in addiction treatment research.
▸Sirtuin activation: In aged mice, NMN administration restored NAD+ to youthful levels, improved mitochondrial function, and increased physical endurance by ~80% vs. untreated aged controls (Gomes et al., 2013; PMID: 24231807).
▸Cognitive function: NMN supplementation in 12-month-old mice improved spatial learning and memory performance; proposed mechanism involves cerebrovascular NAD+ restoration.