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Longevity & Mitochondrial

SS-31 Protocol

Dosing & Research

Last Updated: September 4, 2026

Also known as: Elamipretide

Chemical Identity
Amino Acid Sequence
D-Arg-Dmt-Lys-Phe-NH2
Molecular Formula
C32H49N9O5
Molecular Weight
639.80 g/mol
CAS Number
736992-21-5

Mitochondrial membrane stabilization, Ischemia/reperfusion studies, Muscle fatigue research, Age-related organ dysfunction

Half-Life
~4 Hours
Administration
Subcutaneous
Frequency
5x Weekly

How It Works

  • Mitochondrial membrane stabilization
  • Ischemia/reperfusion studies
  • Muscle fatigue research
  • Age-related organ dysfunction

Dosing Structure

Dose
0.5–1 mg
Frequency
5x Weekly
Notes
MOTS-C/NAD+ Prep: 5mg 5x per week for 2 weeks, then start MOTS-C or NAD+ cycle. Consider a lower dose of SS-31 on off days to keep mitochondria healthy. Low Dose Maintenance: 500mcg 5x per week. SS-31 dosing protocols vary greatly depending on source. Clinical trial doses were up to 40mg per day for 24 weeks with no indication of desensitization; however, recommend greater caution and avoid exceeding 12 weeks without a 4-8 week break. Inject: Abdomen, thigh (SubQ). Morning for general use; pre-workout for performance.

Example Protocols

Dose: 0.5–1 mg
Frequency: 5x Weekly
Duration: 6-8 weeks
Off Period: Not listed
Calculate dosing →

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Protocol Notes

MOTS-C/NAD+ Prep: 5mg 5x per week for 2 weeks, then start MOTS-C or NAD+ cycle.
Consider a lower dose of SS-31 on off days to keep mitochondria healthy.
Low Dose Maintenance: 500mcg 5x per week.
SS-31 dosing protocols vary greatly depending on source.
Clinical trial doses were up to 40mg per day for 24 weeks with no indication of desensitization; however, recommend greater caution and avoid exceeding 12 weeks without a 4-8 week break.
Inject: Abdomen, thigh (SubQ).
Morning for general use; pre-workout for performance.

Research & Studies

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